Sensitization with Eustrongylides sp. (Nematoda: Dioctophymatidae) antigens induce production of specific IgG and IgE in murine model

Abstract Fish consumption plays an important role in human diet. Hoplias malabaricus, commonly known as traíra, is a freshwater fish widely appreciated several Brazilian states and frequently infected by Eustrongylides sp. fourth-instar larvae (L4). The aim of the present study was to evaluate allergenic potential L4 crude extract (CEE). BALB/c mice were immunized intraperitoneally (IP) 10 μg CEE with 2 mg aluminum hydroxide on days 0 35. Specific IgG IgE antibody levels determined after immunization cellular immunity evaluated assessing intradermal reaction ear pavilion. Epicutaneous sensitization performed dorsal region antigen exposure using Finn-type chamber containing 50 or saline solution, followed evaluation specific levels. IP resulted gradual increase transitory production. Significant thickness observed hypersensitivity reaction. In case epicutaneous route, able induce meaningfully increased antibodies well heightened immunity. Both intraperitoneal contact larval antigens for first time be capable inducing immunological mice.